» » Preparative gel chromatography on Sephadex LH-20 (Chromatographic methods)

Download Preparative gel chromatography on Sephadex LH-20 (Chromatographic methods) fb2

by Hans Henke

  • ISBN: 3778524283
  • Category: Math & Science
  • Author: Hans Henke
  • Subcategory: Chemistry
  • Other formats: lrf azw mobi docx
  • Language: English
  • Publisher: Hüthig (1995)
  • Pages: 617 pages
  • FB2 size: 1643 kb
  • EPUB size: 1831 kb
  • Rating: 4.3
  • Votes: 522
Download Preparative gel chromatography on Sephadex LH-20 (Chromatographic methods) fb2

Although, Sephadex column chromatography yielded a similar separation for 100 . Sephadex LH-20 Gel Chromatography. A glass column with a capacity of 237 mL (32cm long and . cm .

ventional Sephadex LH-20 column chromatographic method(3,4). The method, however, requires a long separation time due to a limited flow rate through the. Sephadex column. was. filled with a slurry of Sephadex LH-20 (Pharmacia, Stockholm, Sweden) in 35%. aqueous acetone and equilibrated with the same solvent, and 100 mg of crude.

Start by marking Preparative Gel Chromatography on Sephadex LH-20 as Want to Read . See a Problem? We’d love your help.

Start by marking Preparative Gel Chromatography on Sephadex LH-20 as Want to Read: Want to Read savin. ant to Read.

By (author) Hans Henke. AbeBooks may have this title (opens in new window).

Sephadex LH-20 Sephadex LH-20 is a liquid chromatography medium .

Sephadex LH-20 Sephadex LH-20 is a liquid chromatography medium designed for molecular sizing of natural products such as steroids, terpenoids, lipids, and low molecular weight peptides (up to 35 amino acid residues). Sephadex LH-20 is characterized by: Unique chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix Easily predicted elution behavior based on the chemical structure of the sample (13, 14) Chemical and physical robustness, see Table 1 Excellent batch-to-batch reproducibility.

Sephadex LH-20 has been used extensively as one means of purification prior to gas-liquid chromatography and mass spectrometry as described in the preceding paper. In some instances, highly purified materials can be obtained after even a single passage through Sephadex. In some instances, highly purified materials can be obtained after even a single passage through Sephadex

It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin. The name is derived from separation Pharmacia dextran. These highly specialized gel filtration and chromatographic media are composed of macroscopic beads synthetically derived from the polysaccharide dextran. A special hydroxypropylated form of Sephadex resin, named Sephadex LH-20, is used for the separation and purification of small organic molecules such as steroids, terpenoids, lipids Fractionation. Exclusion chromatography.

literature from MEDLINE, life science journals, and online books .

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books.

Sephadex G-25 is recommended for the majority of group separations involving globular proteins. 19. Sample preparation. Correct sample preparation is extremely important for gel filtration. Simple steps to clarify a sample before applying it to a column will avoid the risk of blockage, reduce the need for stringent washing procedures and extend the life of the packed chromatography medium.

Sephadex LH-20 is a liquid chromatography media designed for molecular sizing of natural products such as steroids, terpenoids, lipids and low molecular weight peptides (up to 35 amino acid residues)

Sephadex LH-20 is a liquid chromatography media designed for molecular sizing of natural products such as steroids, terpenoids, lipids and low molecular weight peptides (up to 35 amino acid residues). Depending on the chosen solvents, this media can also separate sample components by partition between the stationary and mobile phases.

The crude powder was ultrasonic extracted and extraction was pumped into Sephadex LH-20 column directly to cut the nontarget fractions followed by the second-dimensional high-speed countercurrent chromatography, hyphenated by a six-port valve equipped at the post-end of Sephadex.

The crude powder was ultrasonic extracted and extraction was pumped into Sephadex LH-20 column directly to cut the nontarget fractions followed by the second-dimensional high-speed countercurrent chromatography, hyphenated by a six-port valve equipped at the post-end of Sephadex LH-20 column, for the final purification. The results yielded andrographolide with the amount of . 2 mg and a purity of 9. % in a single step, indicating that the present method is effective to harvest target compound from medicinal plant. Download full-text PDF. Source.



Related to Preparative gel chromatography on Sephadex LH-20 (Chromatographic methods) fb2 books: